Renal Function in Humans DEMO

Introduction I Introduction II
Methods I Methods II Methods III
Control Experiment Water-Loading Experiment Bumetanide Experiment Acetazolamide Experiment Hydrochlorothiazide Experiment Amiloride Experiment

Methods (i)

In this program you will be presented with data from human subjects following specific protocols. The protocols for each experiment are different and will be described when you select an experiment.

However each experiment has certain things in common:

Blood samples are taken

Measure:

  1. plasma concentrations of Na+, K+, Cl-, HCO3-, creatinine
  2. plasma osmolality

Urine samples are taken

Measure:

  1. urine volume
  2. urine osmolality
  3. urine output of Na+, K+, Cl-, HCO3-, creatinine, total solute.

Water is ingested

  1. to maintain normal fluid balance (control experiments).
  2. to water load (water-loading and diuretic experiments).

A diuretic is administered in some experiments

  1. Bumetanide
  2. Acetazolamide
  3. Hydrochlorothiazide
  4. Amiloride